Purpose: The purpose of this experiment was to determine the effect of temperature and the amount of oxygen consumption on germinating peas, non-germinating peas and glass beads.
Hypothesis: If germinating peas, non-germinating peas and glass beads are placed in a respirometer the germinating peas will have a higher rate of oxygen consumption.
Hypothesis: If germinating peas, non-germinating peas and glass beads are placed in a respirometer the germinating peas will have a higher rate of oxygen consumption.
Materials:
3 vials
3 pipettes
3 rubber stoppers
masking tape
washers
beads
germinating peas
non-germinating peas
beakers
graduated cylinder
water bath
3 vials
3 pipettes
3 rubber stoppers
masking tape
washers
beads
germinating peas
non-germinating peas
beakers
graduated cylinder
water bath
Procedure:
Begin the experiment by setting up a water bath at room temperature. Next, find the volume of germinating peas, non- germinating peas and bead, and beads alone. Assemble the three respirometers, soaking 3 cotton balls in KOH (Potassium Hydroxide). Place one cotton ball that has been soaked in KOH in each of the respirometers. Put non-absorbent cotton balls in each respirometer above the KOH soaked cotton balls. The first respirometer will contain germinating peas. The second respirometer will have non- germinating peas and glass beads (The glass will make up the water displacement). The third respirometer will have just glass beads (control). Slightly elevate the respirometers, supporting them with masking tape, for 5 minutes while they equilibrate. Add a washer to each respirometer. Then lower the respirometers into the water bath and take a reading every minute for 10 minutes. Record the data into the table.
Begin the experiment by setting up a water bath at room temperature. Next, find the volume of germinating peas, non- germinating peas and bead, and beads alone. Assemble the three respirometers, soaking 3 cotton balls in KOH (Potassium Hydroxide). Place one cotton ball that has been soaked in KOH in each of the respirometers. Put non-absorbent cotton balls in each respirometer above the KOH soaked cotton balls. The first respirometer will contain germinating peas. The second respirometer will have non- germinating peas and glass beads (The glass will make up the water displacement). The third respirometer will have just glass beads (control). Slightly elevate the respirometers, supporting them with masking tape, for 5 minutes while they equilibrate. Add a washer to each respirometer. Then lower the respirometers into the water bath and take a reading every minute for 10 minutes. Record the data into the table.
Methods Summary Chart:
Results:
Discussion/Conclusion:
A. The data shows that germinating peas do have a higher respiration rate because they have been soaking in water and that has activated the endosperms. Dried peas have the next highest level of respiration and glass beads do not respirate. The data does support my hypothesis.
B. My hypothesis stated that germinating peas will have a higher rate of respiration because the endosperms have been activated which causes a larger consumption of oxygen.
C. During this experiment it was really hard to see the rate of oxygen consumption due to the pipette being under water. I suggest that instead of making "homemade" respirometers it would be wiser to use technology (i.e. electronic respirometers) to get accurate readings of the rate of oxygen consumption.
A. The data shows that germinating peas do have a higher respiration rate because they have been soaking in water and that has activated the endosperms. Dried peas have the next highest level of respiration and glass beads do not respirate. The data does support my hypothesis.
B. My hypothesis stated that germinating peas will have a higher rate of respiration because the endosperms have been activated which causes a larger consumption of oxygen.
C. During this experiment it was really hard to see the rate of oxygen consumption due to the pipette being under water. I suggest that instead of making "homemade" respirometers it would be wiser to use technology (i.e. electronic respirometers) to get accurate readings of the rate of oxygen consumption.